nūhou

Loaʻa nā alkolila Toluidine e like me ka anisodamine a me ka cyperine i nā hana kūlohelohe koʻikoʻi a me ke koʻikoʻi lāʻau lapaʻau.These alkaloid ua loa ʻia ma ka hoʻohana lāʻau hou ʻana o nā mea kanu mua, a me ka microbial heterologous synthesis e pili ana i ka biology synthetic me kahi hoʻolālā hou no ka hoʻomākaukau o ka lāʻau lapaʻau kūlohelohe. huahana.I ka Biosynthesis o n-methylpyrrolidine, l-ornithine i hoʻohana ʻia ma ka mea mua, ke ʻano nui o ka ornithine.

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I ka hana ʻana i nā kikowaena heterologous a me ke synthesis o N - methyl pyrroline chassis XiaoYouLi hui flat plume a me xiao-dong li zhou ka hui noiʻi, hana ka noiʻi noiʻi ma lalo nei: 1) ka ʻōlelo hoʻopukapuka mua i ke kumu o nā lāʻau lapaʻau ka ʻekolu o nā lākaAaO2 amine oxidase a ʻAiʻiola biochemical AaDAO3, punawai a me ka hoʻopili ʻana i ka hui koko coca ornithine decarboxylase EcODC a me nā kumu puna putrescine o ka anisodo tanguticus - N - methyl transferase i kahi pā i loko o vitro enzim catalysis synthesis a me ka screening o EcODC, AtPMT a me AaDAO3 i ka hoʻōla o vitro syntropine o N - methyl pyrroline optimal ; 2) ua hoʻokomo hou ʻia nā ʻōnaehana neʻe ʻekolu i loko o kēia pahu i ka escherichia coli a me saccharomyces cerevisiae e kūkulu i nā seleni chassis kūpono a hoʻokō i ka hoʻāla ʻana. Loaʻa ka loaʻa ʻana o ka pūhui n-methylpyrrolium me ka loaʻa ʻana o ka hua 3.02 a me 2.07mg / L kēlā.3) ma ke kumu o kēia, i mea e hoʻonui hou ai ka hana ʻana, ʻo ka noiʻi pū kekahi i ka hoʻohui i nā huehui a ka huʻu a me ka huehue. ʻO ka hopena metyl pyrroline hoʻokūkū i nā ala e pili ana i nā huina o ka ALD4, ALD5 a me HFD1, i ka manawa like i hoʻomālamalama ʻia ʻo SAM2 ma o kahi hāmeʻa nui synthetic AtPMT catalytic synthesis o cofactors i koi ʻia no ka SAM, a me nā mea he huʻu ragi ma ka N - methyl pyrroline a hōʻea i ka nui o 17.82 mg / L, hoʻohālikelike ʻia me ke kumu kūlohelohe mua i hoʻonui ʻia i ka manawa 8,6.

ʻO kēia noiʻi ka mea mua i hana i kahi cell chassis microbial no ka syntitis heterologous o n-methylpyrrolidine, nāna i hoʻokumu i kahi kumu koʻikoʻi no ka nānāʻana a me ka heterologous synthesis o nā lāʻau lapaʻau alkaloids mua o ka n-methylpyrrolidine.

Kākoʻo ʻia ʻia ka noiʻi e ka zhejiang uniana o ka lāʻau lapaʻau ka lāʻau lapaʻau kuʻuna o China zhang chuanyin a loaʻa kālā ʻia e ka Chinese Academy of Science.The characterization of metabolite profiles and small molekala NMR i kākoʻo ʻia e nā kaomi e ka metomos a me ka pūnaehana ʻenekona hoʻonaʻauao kumu o ke kikowaena no nā lawelawe ʻenehana lehulehu. o ke kikowaena no ka hana maikaʻi ʻana o ka molekula


Nā hola mālama: Mei-28-2020